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DWK Life Sciences
| 800.225.1437 (U.S. & Canada Only) | 856.825.1100 |
www.DWK-LifeSciences.comWHEATON
®
CELLine
™
Bioreactors
Advanced Cell Cultivation Devices
Basic Process Description
6.
Incubate flask and repeat the media change/harvesting cycle
until desired amount of protein is generated.
1.
Warm nutrient medium and prepare upper
membrane by adding a small amount of media to
the media compartment.
2.
Inoculate the cell compartment of the device.
3.
Fill the media compartment.
4.
Incubate in a CO
2
incubator for approximately 3-7 days
depending on the optimum harvesting schedule.
5.
Change media from the media compartment and/or harvest
the cells from the cell compartment.
a. The higher the cell density, the more protein production.
Higher cell densities will require more frequent media
changes.
b. Recommended 3, 5 and 7 day feeding schedules are
available. Schedules can be tailored to meet your
production and timing requirements.