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WHEATON
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Cell Culture
Less Work. Less Purification. More Titer.
Culture
Media (mL)
Cell (mL)
Qty /
Cat. No.
Flask Type
Type
Compartment Size
Compartment Size
Case
WCL1000-1
CELLine 1000
Suspension
1000
15
1
WCL1000-3
CELLine 1000
Suspension
1000
15
3
WCL1000AD-1
CELLine 1000-AD
Adherent
1000
15
1
WCL1000AD-3
CELLine 1000-AD
Adherent
1000
15
3
WCL0350-1
CELLine 350
Suspension
350
5
1
WCL0350-5
CELLine 350
Suspension
350
5
5
CELLine
™
Bioreactors
In an effort to push science forward, WHEATON manufactured a new line of membrane
driven flasks for high density cell culture. The flasks are designed to enhance small
scale bio-production for antibody and protein generation. Conventional in vivo or in
vitro cell culture methods can be laborious, may have low cell density and require
significant purification. CELLine flasks address these three areas of limitation observed
in static tissue culture flasks.
Benefits of CELLine Flask
• Disposable and ready-to-use
• High cell density and high product
concentration
• Reduces operation time
• Decreases use of consumables
• Cost-efficient, space saving, and
stackable
• No additional equipment required for
operation
How does the CELLine Flask Work?
Media Compartment
– The media compartment allows for bulk storage of cell culture
growth medium. This reduces the media refreshing requirement significantly as the
media compartment is fifty times the size of the cell compartment.
Metabolite Regulating Upper Membrane
– The upper dialysis membrane has
a 10 kDa cut off limit. This regulates the flow of metabolites to and from the cell
compartment and retains all proteins in the cell compartment.
Cell Compartment
– The cell compartment provides the ideal area to inoculate and
achieve high density cultures. The compartment concentrates cells, their products,
and limits the requirement for any exogenous growth factors.
Gas Permeable Lower Membrane
– With static cultures, gas transfer rates can be
the limiting factor in high density cultures. The CELLine flask places the cells directly
against the gas permeable membrane to achieve optimal levels of oxygen and carbon
dioxide.
Nutrients
Glucose
Glutamine
Waste Products
Lactate
Ammonium
CO
2
O
2
10kDa Membrane
Cells
Proteins
Media
Compartment
Cell
Compartment
Media Port
Inoculation / Harvest Tube
Pipette Tip Stop
Metabolite Regulating Upper Membrane
Cell Compartment Spacer
Base
Gas Permeable Lower Membrane
Base
Silicone Membrane
Inoculation / Harvest Port
Note: Exploded view of device; unit is packaged fully assembled